Antimicrobial Activity of Silver Nanoparticles Synthesized Using Goat Milk against Pathogens of Selected Vegetables

This study was carried outtodetermine the antimicrobial activity of silver nanoparticles synthesized using goat milk against pathogens of selected vegetables. Synthesis of Silver nanoparticles was done usingGoat milk, and characterized using Ultra Violet-Visible absorption spectroscopy, Fourier-transform infrared spectroscopy (FTIR),X-ray diffraction (XRD) and Transmission Electron Microscopy (TEM). Maximum absorbance of Goat milk synthesized AgNPs was observed at 417 nm, with FTIR peaks at3455 cm−1, 1628 cm−1,1402 cm−1, 1081 cm−1and 517 cm−1, indicating that proteins in Goat milk (GM) were the capping and stabilization molecules involved the synthesis of AgNPs. Transmission electron microscopy analysis showed that the biosynthesized particles were spherical in shape having a size of 10-100 nm, X-ray diffraction (XRD)pattern agreed with the crystalline nature and face-centered cubic phase of AgNPs. Evaluation of the antimicrobial activity of AgNPs synthesized using GM against the indicatorstrains (Staphylococcus aureusCIP9973, Pectobacterium carotovorumPec1, Enterobacter cloacaeAS10, Klebsiella aerogenesOFM28, Proteus mirabilisUPMSD3 and Escherichia coli 2013C-3342) isolated from selected vegetables, was carried out using the Agar diffusion assay at different concentrations of 25, 75 and 100 μl/ml. The present study demonstrated that the AgNPs synthesized using Goat milk have potent biological activities, which can find applications in diverse areas.